Laboratorium farmaceutische biotechnologie (onderzoekseenheid)

Id: UG_FW01V2

Universiteit Gent

DNA Fingerprinting: Optimization of DNA analysis using STR technology. Besides routine DNA typing for the department of justice, our lab performs research in the field of DNA fingerprinting for optimising and improvement of the procedures. We created already our own multiplex of four STR loci and investigated the influence of dactyloscopic powders on DNA analysis. At this moment we are investigating the use of immunomagnetic beads to purify and isolate specific cell types from mixed forensic samples. This way sperm cells could be isolated before DNA extraction avoiding time-consuming differential DNA extraction. Forensic mixtures in general can be avoided when this mixed sample consists of different cell types. Rheumatology and Proteomics: Rheumatology: A close encounter with Proteomics. Proteomics is defined as the large-scale characterisation of the entire protein complement of a cell line, tissue and organism. It provides us with a more global and integrated view of biology by studying all the proteins of a cell rather than each one individually. With the completion of the sequencing of several genomes it became clear that a lot of information could not be obtained by studying genes alone. It is impossible to elucidate mechanisms of disease, ageing and effects of the environment solely by studying the genome. Proteomics can provide us with a powerful tool for studying and understanding disease mechanisms and can actively contribute to the search for new drug targets. Because of their nature of pathology, rheumatoid diseases have and are still extensively studied and looked at from an immunological point of view. However, proteomics is catching up with them! We are currently looking at the proteome of several rheumatoid tissues of different rheumatoid diseases in order to find differences in the expressed protein patterns. This study will provide us a better insight in the pathology of these diseases. Proteome analysis of mouse embryonic fibroblasts (MEFs) by 2D-PAGE and mass spectrometry: Natural killer T (NK T) cells are a recently discovered line of T lymphocytes. There is increasing evidence of their regulatory function in several immune processes. Their differentiation pathway requires the interaction with the lymphotoxin b receptor (LTbR) expressed on stromal cells. This interaction promotes the stromal cell to produce several growth factors. Proteome analysis of different MEFs (LTbR stimulated versus non-stimulated MEF) reveals which proteins are potential growth factors. Obsessive-Compulsive Disorder and variants of genes coding for serotonergic and other structures.

Postadressen:

Harelbekestraat 72, 9000 Gent, BE

Disciplinecodes:

• BIOMEDISCHE WETENSCHAPPEN (B)
  • Geneeskunde, mens en vertebraten
    • Farmacologie, formacognosie, farmacie, toxicologie