Researcher
Jan Gettemans
- Keywords:cytoskeleton, protein expression and purification, Nanobody, click chemistry, cancer cell invasion, actin binding proteins, fluorescent labeling
- Disciplines:Cell signalling, Cellular interactions and extracellular matrix, Cytoskeleton, Cell movement, Transcription and translation, Proteins, Nucleic acids, Membrane structure and transport, Lipids, Structural biology, Medical biochemistry and metabolism not elsewhere classified, Cell growth and development, Molecular and cell biology not elsewhere classified, Intracellular compartments and transport, Posttranslational modifications, Other organic elements and compounds
Affiliations
- Department of Biomolecular Medicine (Department)
Member
From1 Oct 2018 → Today - Department of Biochemistry (Department)
Member
From1 Jan 1993 → 30 Sep 2018
Projects
1 - 10 of 35
- Perturbation of extracellular matrix (ECM)-degrading cellulair devices by means of photoporation-directed nanobody delivery in immune cells and cancer cells.From1 Jan 2024 → TodayFunding: BOF - projects
- Nanobody-assisted precision delivery of the moonlighting host defense protein lactoferrin to combat infections with pathogenic bacteria.From1 Jan 2024 → TodayFunding: BOF - projects
- Perturbing tumor-associated macrophage (TAM) equilibrium using GPNMB/CD206 nanobodies and nanoparticles in breast cancer.From1 Oct 2023 → TodayFunding: BOF - mobility
- Electrochemiluminescent Nanobody-based Immunosensor Reactor Platform for Sensing of Disease Biomarkers (LUMIPLAT)From1 Mar 2023 → TodayFunding: Other governmental enterprises (Federal Level)
- Research towards the prophylaxis of Chlamydia psittaci and avian pathogenic Escherichia coli in poultryFrom16 Jul 2022 → TodayFunding: Ministery of Social Affairs, Public Health and Environment
- Perturbation of apolipoprotein E4 (apoE4) activity in AD pathology using single domain antibodies and targeting them to the brainFrom1 Nov 2021 → TodayFunding: BOF - doctoral mandates
- Perturbation of apolipoprotein E4 (apoE4) activity in AD pathology using single domain antibodies and targeting them to brainFrom1 Jan 2021 → 31 Dec 2023Funding: Foundations, funds and other with scientific goal
- Furan-modified peptide and protein ligands for covalent trapping onto cell-surface receptorsFrom1 Nov 2020 → TodayFunding: FWO fellowships
- Stimulated emission depletion microscopy (STED): Nanoscopy for innovative research in the life sciencesFrom1 May 2020 → TodayFunding: FWO Medium Size Research Infrastructure
- New analytical tools to identify and target pathogenic hexameric AB assemblies in Alzheimer's diseaseFrom1 Jan 2020 → 31 Dec 2022Funding: Foundations, funds and other with scientific goal
Publications
1 - 10 of 63
- Site-specific fluorescent labeling, single-step immunocytochemistry, and delivery of nanobodies into living cells(2022)Series: Methods in Molecular Biology
Authors: Jan Gettemans
Pages: 373 - 393 - Triazolinedione protein modification : from an overlooked off-target effect to a tryptophan-based bioconjugation strategy(2022)
Authors: Klaas Decoene, Kamil Ünal, An Staes, Jan Gettemans, Johan Winne, Annemieke Madder
Pages: 5390 - 5397 - An AKT2-specific nanobody that targets the hydrophobic motif induces cell cycle arrest, autophagy and loss of focal adhesions in MDA-MB-231 cells(2021)
Authors: Tijs Merckaert, Jan Gettemans
- Development and characterization of protein kinase B/AKT isoform-specific nanobodies(2020)
Authors: Tijs Merckaert, Jan Gettemans
- Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging(2020)
Authors: Tim Hebbrecht, Jing Liu, Gaelle Boddin, Chloé Van Leene, Klaas Decoene, Annemieke Madder, Kevin Braeckmans, Jan Gettemans
Pages: 33 - 43 - Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation(2020)
Authors: Jing Liu, Tim Hebbrecht, Toon Brans, Eef Parthoens, Saskia Lippens, Chengnan Li, Herlinde De Keersmaecker, Winnok H. De Vos, Rabah Boukherroub, Jan Gettemans, et al.
Pages: 485 - 495 - Nb-induced stabilisation of p53 in HPV-infected cells(2019)
Authors: Anneleen Steels, Laura Vannevel, Olivier Zwaenepoel, Jan Gettemans
- Use, applications and mechanisms of intracellular actions of camelid VHHs(2018)
Authors: Anneleen Steels, Laurence Bertier, Jan Gettemans, Thomas Böldicke
Pages: 205 - 229 - Nanobodies targeting cortactin proline rich, helical and actin binding regions downregulate invadopodium formation and matrix degradation in SCC-61 cancer cells(2018)
Authors: Laurence Bertier, Tim Hebbrecht, Elien Mettepenningen, Natasja De Wit, Adriaan Verhelle, Jan Gettemans
Pages: 230 - 241 - Reviving old protecting group chemistry for site-selective peptide-protein conjugation(2018)
Authors: Smita Gunnoo, Abhishek Iyer, Willem Vannecke, Klaas Decoene, Tim Hebbrecht, Jan Gettemans, Mathias Laga, Stefan Loverix, Ignace Lasters, Annemieke Madder
Pages: 11929 - 11932