The role of CD36 and COUP-TFII in adipogenesis and adipose tissue related angiogenesis.

Obesity, a risk factor for many diseases, is a major cause of mortality and morbidity in Western-type societies. Development of obesity is associated with extensive modifications in adipose tissue, involving adipogenesis, angiogenesis and extracellular matrix proteolysis. CD36, a member of the class B scavenger receptors, may contribute to the pathogenesis of diabetes and obesity. We have shown that its expression significantly increases during adipocyte differentiation. We also found that COUP-TFII, a transcription factor with a documented role in tumor angiogenesis, positively regulated CD36 expression in endothelial cells. However, its relation to CD36 expression in (pre)adipocytes remains unknown and its role in adipose tissue development controversial. In this project we will investigate the role of the COUP-TFII/CD36 axis in adipogenesis and adipose tissue related angiogenesis, by in vitro and in vivo approaches. In vitro, we will use microvascular endothelial cells (MECs), embryonic stem (ES) cells, induced pluripotent stem (iPS) cells and 3T3- preadipocytes. In vivo, de novo adipose tissue formation and transplantation in mice, as well as models of nutritionally induced or genetically determined obesity will be studied. Unravelling the specific functions of CD36 and identification of the COUP-TFII/CD36 signalling pathways during adipose tissue development may yield novel targets for drug development.

Keywords:CD36, COUP-TFII, Obesity, Adipogenesis, Angiogenesis

Disciplines:Laboratory medicine, Palliative care and end-of-life care, Regenerative medicine, Other basic sciences, Other health sciences, Nursing, Other paramedical sciences, Other translational sciences, Other medical and health sciences