High throughput analysis of biomolecular interactions

In this grant we apply for instruments that allow us to measure interactions between biological macromolecules, like proteins, DNA and lipids. These transient interactions between molecules are thought to be the basis of all cellular processes and thus life itself. The combination of instruments requested here was selected because: (A) It allows to measure not only the strength of the interactions but also the speed at which molecules get together and come apart again. (B) The instruments can characterise interactions in very small sample volumes of complex biological samples such as cell lysates or blood serum, and (C) These instruments work fast so that many samples can be analysed in a short space of time, allowing us to begin mapping the thousands of interactions that take place in a cell.