Researcher

Claudina Perez Novo

Research Expertise:My main research activity includes the management of the PPES- Service Facility for Global Kinase Activity Profiling, providing service to both Belgian and international laboratories. On this task, I have gained unique expertise in the Univ. of Antwerp in the profiling of kinases activity in a wide range of biological samples, including cell lines, tissue, and liquid biopsies from patients. The management of this platform also allowed me to learn several protocols for protein lysate preparation and kinase inhibitor screening protocols. I have profiled samples from mouse colon, and brain tissues, cell lines from glioblastoma, pancreatic cancer, neuroblastoma, multiple myeloma, glioma, T-cell acute lymphoblastic leukemia, and peripheral blood samples from Fragile X syndrome and RANKL treated cervical cancer patients. In parallel, I have acquired knowledge in bioinformatics (more recently in integrating kinome data with other omics techniques) and data mining. Besides, I lead several research projects in the field of molecular mechanisms involving the study of kinase signaling and ferroptosis in T-cell acute lymphoblastic leukemia, neuroblastoma, glioma and multiple myeloma, and the role of Withaferin A on kinase activity changes in the regulation of Tau-phosphorylation in in vitro and in vivo Alzheimer’s disease models.

Keywords:PROFILING OF KINASE ACTIVITY FOR DRUG DISCOVERY, PROFILING OF KINASE ACTIVITY AND PERSONALISED MEDICINE, KINASE ACTIVITY PROFILING, Biomedical sciences (incl. biochemistry)

Disciplines:Medical biotechnology, Laboratory medicine, Molecular and cell biology, Oncology, Neurosciences, Other translational sciences, Other medical and health sciences

Research techniques:Disease and research models: Chronic upper and lower airways inflammation, cancer (multiple myeloma and neuroblastoma), and Alzheimer’s disease in vitro and in vivo models. Molecular and Cell Biology techniques: DNA, total RNA and microRNA isolation from cells, serum and tissue, mammalian cells culture, mammalian tissue culture, single-cell suspension protocols, restriction analysis, Western-Blotting, Dot-Blotting, DNA labelling, ELISA, Luminex, Immunohistochemistry, confocal microscopy, Immune and inflammatory cells culture and isolation, Luminex based technology, Protein isolation and Kinase activity profiling. Cell-based assays for cell death screening, Ferroptosis compound screening. Genetic and gene expression analysis techniques: Genotyping of human genes, DNA sequencing, broad experience in gene expression analysis (reverse transcription, real-time PCR, microarrays, RNA-seq, droplet PCR (essential). Epigenetics related techniques: DNA methylation, methylation-specific PCR and bisulphite sequencing (sample and libraries preparation), microRNA analysis (isolation and quantification of microRNAs from human tissues and serum), fundamental data analysis and data mining.

Users of research expertise:Target groups: - Fundamental research laboratories - Pharmacologic research Laboratories - Drug screening research groups - Drug screening companies - Drug Discovery companies - Biomarker discovery research laboratories and companies - Pharmaceutical Biotech companies Target diseases: - Cancer - Metabolic Diseases - Cardiovascular Diseases - Neurodegenerative Diseases - Autoimmune Diseases - Airway Diseases