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Publication
Genetic lineage tracing of beta cell neogenesis
Book Contribution - Chapter
Genetic lineage tracing is an invaluable tool to demonstrate and measure neogenesis of beta cells from putative precursor cells. Cre-Lox recombination technology can be used for indelible labelling of a cohort of cells and following the fate of these cells and their progeny in animal models. Here, the combination is described of beta-galactosidase enzymatic staining with immunohistochemical staining to demonstrate labelled cells. This technique is performed in tissue cryosections.
Book: Animal models in diabetes research
Series: Methods in Molecular Biology
Pages: 317-322
Number of pages: 6
ISBN:978-1-62703-067-0
Publication year:2012
Keywords:diabetes