Development and in vivo validation of a kit for polyamine transport tracking and quantification

Naturally occurring polyamines play a vital role in a multitude of cellular processes, such as cell proliferation and cellular stress. Both metabolism and uptake/transport ensure tight regulation of intracellular polyamine concentrations. Polyamine transporters are attractive drug targets for a broad range of diseases such as cancer, cardiovascular and neurological disorders. We recently identified ATP13A2 and related isoforms as the long sought for human polyamine transporters that are responsible for cellular polyamine uptake. To monitor polyamine transport, we generated transporter cell models and fluorescent polyamine probes that are not yet commercially available. Here, we will develop commercial kits consisting of optimized polyamine probes and our (patented) cell models to monitor polyamine transport/uptake with standardized protocols. Our toolbox will be useful for biochemical, cellular and potentially in vivo applications for basic research and drug screening and development.

Keywords:chemical probes, cancer, neurodegeneration, cardiovascular disease, polyamine transport

Disciplines:Neurological and neuromuscular diseases, Membrane structure and transport