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Publication

Fast-tracking of single emitters in large volumes with nanometer precision

Journal Contribution - Journal Article

Multifocal plane microscopy allows for capturing images at different focal planes simultaneously. Using a proprietary prism which splits the emitted light into paths of different lengths, images at 8 different focal depths were obtained, covering a volume of 50x50x4 µm3. The position of single emitters was retrieved using a phasor-based approach across the different imaging planes, with better than 10 nm precision in the axial direction. We validated the accuracy of this approach by tracking fluorescent beads in 3D to calculate water viscosity. The fast acquisition rate (>100 fps) also enabled us to follow the capturing of 0.2 µm fluorescent beads into an optical trap.
Journal: Optics Express
ISSN: 1094-4087
Issue: 19
Volume: 28
Pages: 28656 - 28671
Publication year:2020
BOF-keylabel:yes
IOF-keylabel:yes
BOF-publication weight:3
CSS-citation score:2
Authors:International
Authors from:Higher Education
Accessibility:Open