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Measuring Rates of ATP Synthesis

Book Contribution - Chapter

Here, we offer you a high-throughput assay to measure the ATP synthesis capacity in cells or isolated mitochondria. More specifically, the assay is linked to the mitochondrial' electron transport chain components of your interest being either through complex I (with or without a linkage to pyruvate dehydrogenase activity), through complex II, or through the electron transport flavoprotein and complex I (β-oxidation of fatty acids).
Book: METABOLIC SIGNALING
Pages: 97 - 107
Number of pages: 11
ISBN:978-1-4939-8768-9
Publication year:2019
Accessibility:Open