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Bioassay-guided isolation of antiplasmodial and antimicrobial constituents from the roots of Terminalia albida

Journal Contribution - e-publication

Ethnopharmacological relevance Terminalia albida (Combretaceae), widely used in Guinean traditional medicine, showed promising activity against Plasmodium falciparum and Candida albicans in previous studies. Bioassay-guided fractionation was carried out in order to isolate the compounds responsible for these activities. Materials and methods Fractionation and isolation were performed by flash chromatography, followed by semi-preparative HPLC-DAD-MS. The structural elucidation of the isolated compounds was carried out by 1D and 2D NMR as well as HR-ESI-MS. Isolated compounds were evaluated against Plasmodium falciparum, Candida albicans, Staphylococcus aureus and Escherichia coli, and their cytotoxicity against MRC-5 cells was determined. Results Bioassay-guided fractionation of Terminalia albida root resulted in the isolation of 14 compounds (1–14), and their antimicrobial properties were evaluated. Pantolactone (1) (IC50 0.60 ± 0.03 μM) demonstrated significant activity against P. falciparum. Other compounds, including 3,4,3′-tri-O-methyl-ellagic acid (3), the triterpenes arjunolic acid (5), arjungenin (6), arjunic acid (7) and arjunglucoside II (10), and the phenol glycoside calophymembranside-B (14), were less active and showed IC50 values in the range 5–15 μM. None of the tested compound showed antibacterial or antifungal activity. Conclusion These results may explain at least in part the activity of the root extract of T. albida against P. falciparum.
Journal: Journal of ethnopharmacology
ISSN: 0378-8741
Volume: 267
Publication year:2021
Keywords:A1 Journal article
BOF-keylabel:yes
BOF-publication weight:2
CSS-citation score:3
Authors:International
Authors from:Higher Education
Accessibility:Open