Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9

Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype.

Publication year:2021

Keywords:CRISPR-Cas Systems, Cadherins, Female, Heterozygote, Humans, Middle Aged, Induced Pluripotent Stem Cells, Neurodevelopmental Disorders

BOF-keylabel:yes

IOF-keylabel:yes

BOF-publication weight:1

Authors:International

Authors from:Higher Education

Accessibility:Open