Second and Third Harmonics, a dynamic and Label-free imaging technique to study (bio)-polymers and cellular structuresAcronym: STHar-Lite

Microscopic imaging is a powerful tool to investigate dynamic processes in several different research fields. With the advent of pulsed infrared lasers, it is now possible to excite fluorescent molecules present deep in living tissues. These non-linear multiphoton approaches also enable harmonic light generation not only in a variety of chemicals but also in a number of biomolecules such as collagen, myosin and microtubules. However, harmonic imaging is not yet a widespread technique, mainly due to the lack of in-depth knowledge on how second and third harmonic observables come about, especially in tissues. A thorough understanding of the molecular and interface aspects of harmonic light generation is essential to correctly interpret the images generated. In this proposal, four scientists with different backgrounds and complementary interests in optics and imaging, will use join forces to advance the understanding of harmonic imaging and broadening its usability.

Keywords:microscopy, second and third harmonics, neuronal imaging, cardiac myocyte, molecular photonics

Disciplines:Photonics, optoelectronics and optical communications, Optical properties of materials, Cardiology, Neurophysiology