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Project

Genetic characterization of the S-locus in pear (Pyrus communis): exploiting haplotype diversity to characterize the functional role of SFBBs in the S-RNase-based GSI system

Pear, like many other Rosaceae species, exhibits an S-RNase-dependent gametophytic self-incompatibility system (GSI) that inhibits fertilization by self- or cross-incompatible pollen. The GSI system in Pyrus species is regulated by a polymorphic S-locus which contains a stylar expressed S-RNase gene and multiple S-locus F-box (SFBB) genes that are expressed in the pollen. However, despite extensive characterization of the female S-RNase factor, little is yet known about the identity and functioning of the SFBB male S-determinants, nor about their specific interaction with the various S-RNases. The aim of proposed research project is therefore to identify all SFBB genes in the Pyrus S-locus, and to take advantage of natural genetic variation in S-haplotypes to unravel the complex interaction between SFBBs and their S-RNase targets. Following initial identification of putative SFBB genes based upon long-read nanopore sequencing of the genome of the two Pyrus communis cultivars “Bartlett” and “Conference”, and assembly of the S-locus haplotypes, we will validate and functionally characterize predicted S-RNAse-SFBB interactions using a combination of dedicated in vitro and in vivo assays (Y2H, CRISPR gene editing, etc.). Results of this study will greatly contribute to our understanding of the genetic determination and molecular functioning of the GSI system in pear, with major relevance for future plant reproductive research as well as horticultural applications.

Date:1 Oct 2022 →  Today
Keywords:Self-incompatibility, Pyrus, SFBB
Disciplines:Plant developmental and reproductive biology, Crop science, Agricultural plant breeding and biotechnology, Horticultural crop production