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Project

Detailed investigation of eddy dispersion in hydrophilic interaction liquid chromatography and reversed-phase liquid chromatography under conditions of identical packing structure

High-performance liquid chromatography (HPLC) is currently the dominant analytical tool in many areas. It is used extensively in the pharmaceutical industry for applications ranging from content uniformity assays to pharmacokinetic studies, and is used widely in environmental laboratories for the analysis of pesticides and other contaminants in soil and water. In clinical analysis, therapeutic drug monitoring is also performed by HPLC. Therefore, any new development in HPLC is expected to have a large impact on a wide variety of fields. Most separations in HPLC are performed under reversed-phase liquid chromatography (RPLC) conditions. A broad range of compounds can be separated using this separation mode. However, when polar and ionizable compounds (e.g., metabolites and degradation products) need to be analyzed, hydrophilic interaction liquid chromatographic (HILIC) columns can be an interesting alternative. It has been demonstrated that band broadening phenomena in RPLC and HILIC are inherently different, and can lead to decreased column performance under HILIC conditions. In this proposal, a thorough fundamental investigation of the band broadening phenomena, and more specifically eddy dispersion, contributing to the overall column efficiency under HILIC and RPLC conditions will be made. Insights gained during this investigation can be used to make specific recommendations on column design to obtain more performant columns for HILIC separations in the future.

Date:1 Oct 2017 →  1 Dec 2019
Keywords:Eddy dispersion
Disciplines:Sustainable chemistry