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Project

Mechanistic study of zwitterionic hydrophilic interaction liquid chromatography

Zwitterionic hydrophilic interaction liquid chromatography (HILIC) stationary phases have gained increasing attention in pharmaceutical analysis in recent years due to their specific selectivity for polar compounds. Although well advanced, the retention mechanism of HILIC is still not fully resolved and rather complex. The main purpose of this PhD project is to prepare and study novel HILIC capillary columns, to better understand their retention mechanism and guide their applications in pharmaceutical analysis. The principal research work is as follows: 1. Preparation of different novel zwitterionic HILIC capillary columns, 2. Chromatographic characterization of zwitterionic HILIC capillary columns, 3. Systematic investigation of the effect of sample solvent on peak shape in capillary hydrophilic interaction liquid chromatography, 4. Kinetic performance assessment of different in-house made capillary columns and comparative study with commercial columns, 5. Applications of zwitterionic HILIC capillary columns in pharmaceutical analysis.

Date:11 Sep 2019 →  17 Jul 2023
Keywords:Chromatography, Pharmaceutical analysis
Disciplines:Analytical separation and detection techniques
Project type:PhD project