Multi-dimensional liquid chromatography for the analysis of complex samples.

Funding is requested for the acquisition of a multi-dimensional liquid chromatography instrument for the analysis of the increasingly complex samples the consortium is currently confronted with. These complex samples consist of a large number of compounds, with a wide variety in physicochemical properties that are very difficult to separate on a single separation column. Multi-dimensional liquid chromatography allows to combine multiple, orthogonal separation columns in a single analysis, allowing to analyze compounds with very different properties simultaneously. Commercial equipment to perform multi-dimensional analyses has only recently become available. It is the ambition of the consortium to demonstrate its potential by applying it for the analysis of samples from diverse fields of life sciences and engineering technology, while at the same time developing new software strategies for the automated use of this equipment.

Keywords:liquid chromatography, complex samples, multidimensional analysis, environmental analysis, pharmaceutical analysis

Disciplines:Analytical separation and detection techniques, Chemical process design