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Identification of EPS-degrading activity within the tail spikes of the novel Pseudomonas putida phage AF

Journal Contribution - Journal Article

We report the study of phage AF, the first member of the canonical lambdoid phage group infecting Pseudomonas putida. Its 42.6 kb genome is related to the "epsilon 15-like viruses" and the "BPP-1-like viruses", a clade of bacteriophages shaped by extensive horizontal gene transfer. The AF virions display exopolysaccharide (EPS)-degrading activity, which originates from the action of the C-terminal domain of the tail spike (Gp19). This protein shows high similarity to the tail spike of the T7-like P. putida-infecting phage phi 15. These unrelated phages have an identical host spectrum and EPS degradation characteristics, designating the C-terminal part of Gp19 as sole determinant for these functions. While intact AF particles have biofilm-degrading properties, Gp19 and non-infectious AF particles do not, emphasizing the role of phage amplification in biofilm degradation. (C) 2012 Elsevier Inc. All rights reserved.
Journal: VIROLOGY
ISSN: 0042-6822
Issue: 2
Volume: 434
Pages: 251 - 256
Publication year:2012
Keywords:Virology, bacteriophage, P.putida, exopolysaccharide-degrading enzyme, biofilm degradation, horizontal gene transfer, Bacteriophage, P. putida, Exopolysaccharide-degrading enzyme, Biofilm degradation, Horizontal gene transfer
BOF-keylabel:yes
IOF-keylabel:yes
BOF-publication weight:1
CSS-citation score:2
Authors:International
Authors from:Higher Education
Accessibility:Closed