A simple procedure for the enzymic digestion of edible tissues prior to detection of drug residues

A simple procedure for the enzymic digestion of edible tissues is described and compared with other procedures. The samples are digested overnight in an enzyme suspension containing subtilisin A at 60 degrees C and pH 9. The resulting digest contains only a few small tissue fragments. This method is suitable for routine analysis, since the manipulation of the samples is very limited.

Keywords:Animals, Cattle, Drug Residues, Meat, Microbial Collagenase, Muscles, Subtilisins