Minimizing metabolic burden caused by heterologous expression of synthetic pathways: development of sensors & actuators, implementation of a genetic circuit with beta-carotene production in E. coli as case study Ghent University
The expression of synthetic multi-gene pathways in non-native production hosts often results in metabolic burden, which is detrimental for productivity. A novel genetic circuit will be developed which specifically controls the expression of the heterologous pathway, so that metabolic burden can be avoided. This novel synthetic biology tool will be validated using beta-carotene production in E. coli as case study.