Projects
MOLECULAR DESIGN OF U+2018FROZENU+2019 APTAMERS: Aptamers with enhanced properties by covalent and non-covalent stabilization using functionalized nucleotides: a combined modelling, NMR and electrochemistry approach Ghent University
De on-site en zeer selectieve detectie van cocaïne en andere belangrijke doelanalyten zoals
antibioticaresiduen in afvalwater blijven een uitdagend onderzoeksgebied. In dit project willen we dat
beter begrijpen en uiteindelijk verbeteren van analytische detectiesystemen die kort DNA gebruiken
sequenties voor selectieve herkenning van interessante moleculen - aptamers genaamd - gevolgd door de
generatie van elektrische ...
Aptamer micro-factories: replacing SELEX by a single-step aptamer selection strategy. KU Leuven
Aptamers are single stranded oligonucleotides that, similarly to antibodies, bind their targets with great affinity and specificity by adopting a defined 2D/3D structure. Aptamers have been widely employed as target recognition molecules in diagnostics and are currently being introduced in the clinic as therapeutics. Isolation of new aptamers is a non-trivial process relying on an in vitro method termed SELEX, based on the iterative ...
Aptamer micro-factories: replacing SELEX by a single-step aptamer selection strategy KU Leuven
Developments in medical diagnostics, environmental monitoring, food safety and therapeutics require systems that make it possible to detect a wide range of molecules in a fast and easy, yet specific and sensitive manner. In light of these applications and driven by this demand, the field of biosensing has demonstrated huge potential. In spite of this potential, continuous improvements beyond the state of the art are necessary to help overcome ...
Crosslinking Aptamers for Covalent Protein Targeting Ghent University
Aptamers are single-stranded nucleic acid ligands that bind targets with high affinity and specificity, owing to their characteristic three-dimensional structures. Because of these features, aptamers have been widely studied in different applications, including targeted therapy, cell staining and biosensors. Nevertheless, the translation into real-world clinical and analytical practice is still very limited, underlining the need for further ...
Evolve HNA aptamers by Cell-SELEX as molecular tools for cancer study. KU Leuven
Aptamers are single-stranded DNA and RNA molecules. They can bind to diverse molecular targets with high affinity and specificity by folding unique three dimensional structures. Therefore, aptamers are useful tools for therapeutic and diagnostic purposes. However, aptamers made of DNA and RNA are endowed with low biostability and poor pharmacodynamices, limiting their use for clinical development. In this study, we propose to develop a ...
Structure and folding of XNA aptamers studied by computational and experimental methods KU Leuven
Aptamers are folded oligonucleotides that bind to a specific target with a high affinity and selectivity. Originally they were composed of natural nucleic acids (RNA or DNA) that are identified in a large library through a process termed Systematic Evolution of Ligands by EXponential enrichment (SELEX). Since natural nucleic acids have short half-life in biological conditions, research shifted to aptamers composed of artificial (xeno) nucleic ...
Selection of nucleic acids aptamers with four non-canonical bases for biomedical applications KU Leuven
New accurate and cost-effective technologies are required for precision detection and analysis of human disorders and environmental biomarkers. To this end, aptamers, specific nucleic acid sequences that tightly bind to a broad range of biomolecules (e.g., proteins, toxins, antibiotics), demonstrate great potential. They have quickly become alternatives to protein-based agents, antibodies, in various applications including diseases treatment ...