In vivo monitoring of DNA modification. Redesign of an E. coli DNA repair protein for sensing specific DNA modifications. KU Leuven
In this thesis, we aimed to create specific binders for 1-methyl adenine (1mA) in methylation-damaged DNA. Such binders may have potential as biosensor of DNA methylation lesions as the current experimental state-of-the- art does not allow for in vivo detection of methylated DNA. To that end, we initiated the molecular evolution of the E. coli AlkB (EcAlkB) DNA repair protein, a prototypical and promiscuous demethylase known ...