Projects
Research Equipment: BD LSRFortessa TM flowcytometer Ghent University
The research of both the 'Laboratory for Translational Research in Obstructive Pulmonary Diseases' as the 'Tumor Immunology Lab' encompasses a critical mass of activities that warrants the isntallation of a new, high-performance flowcytometer within our direct research environment.
Beckman Coulter Gallios flowcytometer. KU Leuven
Within the research of the beta cell and immune system, it is essential to use flow cytometry technology. This is located at different levels. First and foremost, there is a need for translation of findings from the RNA and even proteomics area, in which general directional findings pave the way. The flow cytometry equipment allows to confirm or refute findings at protein level. It also offers the great added value that one can locate the ...
Alternative induction of polyploid ornamentals and their added value Research Institute for Agriculture, Fisheries and Food
Polyploids are plants with more than two chromosome sets. They can be induced relatively easily with chemicals; however, this technique leaves us with some questions. Can we develop new technology to create polyploids and genetic variation in ornamentals? Can we quantify and increase the synthesis of gametes with an increased number of chromosome sets (2n or unreduced gametes)? Is it possible to ...
Impedance flow cytometry with specificity towards epitopes KU Leuven
The proposed research is on development of purely electrical flow-cytometer. Cytometer is an instrument that is capable of assessing cell morphology (size, granularity), and detect presence of characteristic epitopes (antigenes or receptors) on the cell membrane. Investigated cells are flowing consecutively through the sensor allowing to quickly classify the biological sample, for instance, blood cells. High speed and high sensitivity make ...
A flow cytometer to perform multiparameter sorting, including single-cell deposition under biohazard conditions L2 Ghent University
Flowcytometry and cell sorting is indispensable for research in cellular immunology. Using flowcytometry, it is possible to discriminate and isolate morphologically identical cells based on the expression of a set of membrance proteins. This technique will be used to analyse and isolate T and NK cells and precursors thereof and to sort experimentally infected HIV cells.
Ultra-sensitive minimal residual disease assessment by blood-based clonotypic mass spectrometry approach in plasma cell dyscrasias (MASSIAS) KU Leuven
With nearly 1000 new diagnoses in Belgium in 2020, multiple myeloma accounted for ~1.5% of all new registered cancer diagnoses in that year. Current response criteria in treated multiple myloma patients recommend determining minimal residual disease (MRD) detection with either next-generation flowcytometry (NGF) or next-generation sequencing (NGS) on bone marrow aspirates as the cornerstone of disease management in patients in complete ...
Elucidating the immunomodulatory role of sex hormones in asthma Ghent University
Before puberty, asthma prevalence is highest in boys but there is a sharp decrease during puberty. Two thirds of adult asthma patients are female, who typically have a more severe phenotype and a lower quality of life compared to men. In addition to genetic and environmental factors these data suggest a pivotal role of sex hormones, namely testosterone (T), in the pathogenesis of asthma.
While epidemiologic and observational studies ...
Sjögren's syndrome pathophysiology investigating trial: the SSPIT concept Ghent University
Primary Sjögren’s syndrome (pSS) is a common autoimmune systemic rheumatic disease with an unrevealed pathophysiology, affecting around 1-2% of the population. The main presenting symptoms are ocular and oral dryness. In addition to these sicca symptoms, systemic features with more serious complications affecting joints, skin, lungs, and peripheral nervous system are seen in 15% of pSS patients. Furthermore, about 5% of patients develop a B ...
Cofunding core facility - Flow cytometry Ghent University
A basic Flow cytometry infrastructure available to every UGent researcher, SOC' researcher and external researchers that, through immediately available training and guidance, allows in a quality (accurate and reproducible) manner both basic (pre-analytical) analyses and in-depth innovative immunological analyses and cell sorting in the most optimal financial and operational conditions.