Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs) Research Institute for Agriculture, Fisheries and Food
For the quantification of genetically modified organisms (GMOs) in foods and feeds, real-time PCR is currently the most widely applied technique. To obtain a % of GMO, a GMO-specific target sequence is quantified relatively to a species-specific sequence. The correctness and reliability of the obtained quantitative results fully depend on the reference materials used as standards for setting up external calibration curves. We introduced a ...