Rivers act as a natural source of greenhouse gases (GHGs). However, anthropogenic activities can largely alter the chemical composition and microbial communities of rivers, consequently affecting their GHG production. To investigate these impacts, we assessed the accumulation of CO2, CH4, and N2O in an urban river system (Cuenca, Ecuador). The study area is located at the Cuenca River basin situated in the southern province of Azuay in the Andes of Ecuador. The basin is composed of five main tributaries, i.e. Cuenca, Tarqui, Yanuncay, Tomebamba, and Machangara Rivers. The study area is 572.92 ...