Quantitation of Chronic and Acute Treatment Effects on Neuronal Network Activity Using Image and Signal Analysis: Toward a High-Content Assay KU Leuven University of Antwerp
Upon maturation, primary neuronal cultures form an interconnected network based on neurite outgrowth and synaptogenesis in which spontaneous electrical activity arises. Measurement of network activity allows quantification of neuronal health and maturation. A fluorescent indicator was used to monitor secondary calcium influxes after the occurrence of action potentials, allowing us to examine activity of hippocampal cultures via confocal live ...