Publications
Site-specific fluorescent labeling, single-step immunocytochemistry, and delivery of nanobodies into living cells Ghent University
Sortase A-mediated site-specific labeling of camelid single-domain antibody-fragments Vrije Universiteit Brussel Ghent University
A generic site-specific conjugation method that generates a homogeneous product is of utmost importance in tracer development for molecular imaging and therapy. We explored the protein-ligation capacity of the enzyme Sortase A to label camelid single-domain antibody-fragments, also known as nanobodies. The versatility of the approach was demonstrated by conjugating independently three different imaging probes: the chelating agents CHX-A"-DTPA ...
Site-specific labeling of 'second generation' annexin V with Tc-99m(CO)(3) for improved imaging of apoptosis in vivo KU Leuven University of Antwerp
Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis KU Leuven
Site-specific labeling of his-tagged Nanobodies with 99mTc: A practical guide Vrije Universiteit Brussel
Singlet oxygen-induced furan oxidation for site-specific and chemoselective peptide ligation Ghent University
Site-specific ⁶⁸Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models University of Antwerp
Rapid tumor-labeling kinetics with a site-specific near-infrared anti-CEA nanobody in a patient-derived orthotopic xenograft mouse model of colon cancer Vrije Universiteit Brussel
Background/Objectives: Nanobodies are the smallest biologic antigen-binding fragments derived from camelid-derived antibodies. Nanobodies effect a peak tumor signal within minutes of injection and present a novel opportunity for fluorescence-guided surgery (FGS). The present study demonstrates the efficacy of an anti-CEA nanobody conjugated to near-infrared fluorophore LICOR-IRDye800CW for rapid intraoperative tumor labeling of colon cancer. ...