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A viability quantitative PCR dilemma : are longer amplicons better? Ghent University KU Leuven
Endothelial Cell Viability after DMEK Graft Preparation Vrije Universiteit Brussel
Aim: To evaluate the effect of graft preparation and organ-culture storage on endothelial cell density (ECD) and viability of Descemet membrane endothelial keratoplasty (DMEK) grafts. Materials and methods: DMEK grafts (n = 27) were prepared at Amnitrans EyeBank Rotterdam from 27 corneas (15 donors) that were eligible for transplantation but could not be allocated due to the Covid-19-related cancellation of elective surgeries. Cell viability ...
Utilizing osteocyte derived factors to enhance cell viability and osteogenic matrix deposition within IPN hydrogels Ghent University
Many bone defects arising due to traumatic injury, disease, or surgery are unable to regenerate, requiring intervention. More than four million graft procedures are performed each year to treat these defects making bone the second most commonly transplanted tissue worldwide. However, these types of graft suffer from a limited supply, a second surgical site, donor site morbidity, and pain. Due to the unmet clinical need for new materials to ...
Viability of community forests as social enterprises : a Cameroon case study Ghent University
Since the concept of community forests was instituted in Cameroon in 1994, there has been an upsurge of such forest management arrangements in the country. However, up to now there is no conclusive evidence as to whether such schemes can operate as profitable ventures and at the same time meet their social and environmental objectives. The latter is the core objective of a social enterprise that constitutes the basis of our analysis. In fact, ...
Assessing the viability of a synthetic bacterial consortium on the in vitro gut host-microbe interface Ghent University
The interplay between host and microbiota has been long recognized and extensively described. The mouth is similar to other sections of the gastrointestinal tract, as resident microbiota occurs and prevents colonisation by exogenous bacteria. Indeed, more than 600 species of bacteria are found in the oral cavity, and a single individual may carry around 100 different at any time. Oral bacteria possess the ability to adhere to the various niches ...
Assaying Cellular Viability Using the Neutral Red Uptake Assay Vrije Universiteit Brussel
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration-dependent reduction of the uptake of neutral red after exposure to the xenobiotic under investigation. The neutral red uptake assay is mainly used ...