Publications

We present an optimized electron activated dissociation (EAD) methodology, based on hot electron capture dissociation, for liquid chromatography-tandem mass spectrometry characterization of N- and O-glycopeptides, using recombinant human erythropoietin as a model glycoprotein. Applying a full factorial design of experiments (DoE) approach, we first optimized LC-MS parameters (i.e., ion spray voltage, ion source temperature, and active gradient ...

Tissue spheroids fuse to form larger tissue structures, a process controlled by their living material properties. However, how these properties emerge from the active behavior of individual cells is not well understood. Here, we studied fusion dynamics of spheroids from human periosteum-derived cells. Using confocal microscopy, we measured spheroid granularity and, with two-photon microscopy, we quantified active cell movements during fusion. ...

Enhancing the structural stability and electronic and electrochemical properties of sodium-ion cathode materials is essential for developing the next generation of sodium-ion batteries. In this study, we carry out first-principles calculations to assess the performance of P2-type sodium lithium manganese oxide (NLM) cathodes co-doped with transition metals X (X = Sc, Ti, V, Cr, Fe, Co, Ni, Cu, and Zn) and fluorine. Our findings reveal that among ...

System xc- (with xCT as specific subunit) is an astrocytic cystine/glutamate antiporter that constitutes the major source of extracellular glutamate in the mouse striatum. We previously reported that young-adult mice lacking xCT (xCT-/- mice) display decreased intracellular glutamate levels in pre- and post-synaptic compartments at corticostriatal synapses as well as impaired corticostriatal neurotransmission, compared to wildtype (xCT+/+) ...

Intensive care unit acquired weakness (ICUAW) is common in critically ill patients, contributing to substantial morbidity. Major trials and novel mechanistic findings published over the past years have advanced knowledge for the prevention and treatment of ICUAW. To streamline future research priorities, a multinational, interdisciplinary group of ICU clinicians, researchers, and people with lived experience convened to develop this ...

BACKGROUND: In the PAPILLON study, first-line amivantamab-chemotherapy in epidermal growth factor receptor (EGFR) exon 20 insertion-mutated non-small cell lung cancer demonstrated significantly prolonged progression-free survival and favorable overall survival over chemotherapy; a consistent benefit was also observed across some secondary endpoints. However, the complete clinical benefit of first-line amivantamab-chemotherapy is not fully ...

Information and Communication Technologies (ICTs) significantly altered our working lives. They advanced effectiveness and flexibility, yet also fuel techno-invasion, techno-overload and decrease employee well-being. However, despite booming scientific research on ICTs use by employees, remarkably little is known about non/limited use. Therefore, this project develops insight in digital disconnecting to decrease negative outcomes while ...

Advanced atomistic computations have been applied to investigate the structural, electronic, and transport properties of the unexplored chloride compounds Na2M3Cl8 with M = Mg, Zn, Ca, and Sr. Lattice parameters computed using density functional theory and force field methods closely match reported values, particularly when compared to experimental values of Na2Mg3Cl8. Electronic structure analysis confirms that Na2M3Cl8 exhibits semiconductor ...