Projects
Novel microscope designs for fast and highly adaptable fluorescence microscopy. KU Leuven
The development, screening and characterization of novel fluorescent probes for applications in fluorescence microscopy with nanometer resolution. KU Leuven
In situ investigation of biochemical and nanocolloidal dynamics using fast optical nanoscopy: Video rate high resolution fluorescence microscopy in material science and biochemistry. KU Leuven
Nanomaterials are all around us. They are widely used as they show some extraordinary properties as catalysts (showing unexpected reactivity or needing less starting material), as additives to change properties of bulk materials (exhibiting luminescent properties like in quantum dots, or altering wetting behavior for self cleaning effect), biomedical applications for target drug delivery, potential investigation tool or new treatment ...
Super resolution fluorescence microscopy of biomolecular interactions with photochromic biosensors and complementation. KU Leuven
Fluorescence microscopy techniques are indispensable research tools that have enabled live cell imaging with low invasiveness, high sensitivity, and high spatiotemporal resolution. As a result, fluorescence microscopy has revolutionized our understanding of the inner workings of single cells and whole organisms. An integral part of the imaging performance is the fluorescent labels, as the quality of the labels influences the quality of the ...
Visualization of biomolecular interactions and sensing in living systems with super-resolution fluorescence microscopy. KU Leuven
Fluorescence microscopy is a powerful method to study living systems with high spatial and temporal resolution. However, the resolution of a conventional microscope is limited by diffraction, which precludes the direct visualization of many biological processes occurring at their small scale. During the last decade, several super-resolution fluorescence microscopy methods have been developed that break this diffraction limit and offer a new ...
Fluorescence microscopy tools for in situ catalyst characterization KU Leuven
The goal of this PhD thesis is to apply fluorescence microscopy to investigate the interplay between local catalytic performance and catalyst porosity and to derive structure-activity and selectivity relationships for heterogeneous catalysts at the level of individual catalytic turnovers. To achieve this goal, I employed both diffraction-limited and super-resolution fluorescence microscopy with support from other techniques.
A first ...
Multimodal fluorescence microscopy and nanoscopy platform. KU Leuven
Fluorescence microscopy and super-resolution fluorescence microscopy -nanoscopy- offers the possibility to study in a non-invasive way structures ultimately down to the nanometer scale. The ability to do so dynamically will prove to be an indispensable tool in analyzing the molecular mechanisms and organization of living as well as non-living systems. Technological advances in the microscopy field have now resulted in commercially available ...
Advanced illumination techniques for high-contrast highresolution fluorescence and scattering microscopy Ghent University
In light microscopy an object is illuminated with a lamp or a laser and the transmitted,
reflected or fluorescent light is detected to get information on the object. In the project two approaches will be developed to study small particles and their interaction with living cells: Light Sheet Fluorescence Microscopy and Complex Beam Scattering Microscopy.
In standard fluorescence microscopy the entire sample is illuminated and there ...
High Resolution Structured Illumination Raster Scanning Fluorescence Microscopy KU Leuven
Fluorescence microscopy has become an indispensable tool in
biology and medicine. It is routinely used in drug development, to
diagnose health disorders and in DNA sequencing.
Despite enormous progress in instrumentation and deployment, high-
resolution microscopes remain expensive, bulky devices that require
skilled operators. As a consequence, they are found only in
specialized labs, limiting their ...