Projects
White light laser-based confocal fluorescence lifetime imaging microscope for multi-parameter 3D live imaging Ghent University
Fluorescence lifetime imaging microscopy (FLIM) is anemerging tool for studying biological processes in live,multi-parameter and quantitative manner. Modern FLIM-compatible confocal microscopes equipped with respectivehardware and software enable for fast and high-quality 3Danalysis of cell and tissue oxygenation, metabolism, cellproliferation and differentiation, their response to therapyand infection. While already able addressing ...
The development of novel quenched fluorescent activity-based probes and their application in the visualization of serine protease activity in living cells by microscopy and flow cytometry KU Leuven
Proteases are enzymes that cut proteins. Although some play a role in food digestion, most are involved in the regulation of specific biological processeses in the body. Aberrant protease activity contributes to the development of diseases, and proteases can therefore be used as drug targets and diagnostic markers. For example, some tumor types display an increased protease activity. If we were able to localize a particular protease and show ...
Total internal reflection fluorescence microscope (TIRF microscope). KU Leuven
Combined X-Ray Fluorescence and Absorption CT (XRF-CT) imager for non-destructive 3D elemental/structural analysis on the microscopic scale. Ghent University
In several scientific disicplines (environmental and life sciences, toxicology, cosmo chemistry, geochemistry, archeology, electronics), there is an increasing demand for the development of non-destructive techniques for spatial distribution of elements within the material to be examined, along with the internal morphology thereof on the micrometer scale, may be determined in situ. The recent developments in the field of micro-XRF and ...
Integrated fluorescent microscope infrastructure for physical and biological applications Ghent University
Integrated system for microscopic studies of living celsystems and ultra small fluorescent particles.
A correlative microscopy approach for intracellular structures under cryogenic conditions based on Single-Molecule Localization Microscopy and X-ray nanoscopic imaging. Ghent University
Super-resolution microscopy techniques allow the visualization of antibodies - markers for locating intracellular and extracellular proteins - with a resolution of <10 nm, allowing the visualization of the internal structure of cells. Stochastic recently developed, relatively inexpensive, but powerful super-resolution microscopy techniques. project, we want to build a cost-efficient dual wavelength STORM microscope (the setup can ...