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Real-time PCR detection and quantification of selected transferable antibiotic resistance genes in fresh edible insects from Belgium and the Netherlands KU Leuven
The occurrence of antibiotic resistance genes in foodstuffs involves a human health risk. Fresh edible insects present an emerging source of human food but they were not yet assessed in a quantitative way for antibiotic resistances as a matter of food safety. In this study, a real-time quantitative PCR assessment was optimised to detect and quantify relevant transferable antibiotic resistance genes [tet(O, K, M, S) and erm(B)] in edible insects. ...
Identification of bacterial endospores and targeted detection of foodborne viruses in industrially reared insects for food KU Leuven
With edible insects being increasingly produced, food safety authorities have called for the determination of microbiological challenges posed to human health. Here, we find that the bacterial endospore fraction in industrially reared mealworm and cricket samples is largely comprised of Bacillus cereus group members that can pose insect or human health risks. Hepatitis A virus, hepatitis E virus and norovirus genogroup II were not detected in ...
Intraguild predation by Harmonia axyridis (Coleoptera: Coccinellidae) on native insects in Europe: molecular detection from field samples Ghent University
Reference gene selection for insect expression studies using quantitative real-time PCR: The head of the honeybee, Apis mellifera, after a bacterial challenge KU Leuven Ghent University
In this study an important and often neglected aspect of gene expression studies in insects, the validation of appropriate reference genes with stable expression levels between sample groups, is addressed. Although in this paper the reference gene selection for the honeybee, Apis mellifera L. (Hymenoptera: Apidae) head was tested in the context of bacterial challenge with Escherichia coli, this work can serve as a resource to help select and ...
Keeping your armour intact : how HIV-1 evades detection by the innate immune system : HIV-1 capsid controls detection of reverse transcription products by the cytosolic DNA sensor cGAS Ghent University
HIV-1 infects dendritic cells (DCs) without triggering an effective innate antiviral immune response. As a consequence, the induction of adaptive immune responses controlling virus spread is limited. In a recent issue of Immunity, Lahaye and colleagues show that intricate interactions of HIV capsid with the cellular cofactor cyclophilin A (CypA) control infection and innate immune activation in DCs. Manipulation of HIV-1 capsid to increase its ...
A novel tau antibody detecting the first amino-terminal insert reveals conformational differences among tau isoforms University of Antwerp KU Leuven Vrije Universiteit Brussel
As human Tau undergoes pathologically relevant post-translational modifications when expressed in yeast, the use of humanized yeast models for the generation of novel Tau monoclonal antibodies has previously been proven to be successful. In this study, human Tau2N4R-Delta K280 purified from yeast was used for the immunization of mice and subsequent selection of high affinity Tau-specific monoclonal antibodies. The characterization of four novel ...
Machine Translation for Multilingual Intent Detection and Slots Filling University of Antwerp
We expect to interact with home assistants irrespective of our language. However, scaling the Natural Language Understanding pipeline to multiple languages while keeping the same level of accuracy remains a challenge. In this work, we leverage the inherent multilingual aspect of translation models for the task of multilingual intent classification and slot filling. Our experiments reveal that they work equally well with general-purpose ...
Optimised dynamic line scan thermographic detection of CFRP inserts using FE updating and POD analysis Vrije Universiteit Brussel University of Antwerp
The detection of delaminations in composite laminates using automated thermographic scanning is a quite challenging task. The set-up parameters are not only dependent on the equipment, but on the inspected component as well. In this work, a methodology is discussed to use Finite Element (FE) model updating to automatically establish the most suitable inspection parameters for a given combination of the structure and the investigated ...
Intact animacy perception during chase detection in ASD University of Antwerp KU Leuven
We explored the strength of implicit social inferences in adolescents with and without Autism Spectrum Disorder (ASD) using a chasing paradigm in which participants judged the absence/presence of a chase within a display of four seemingly randomly moving dots. While two of these dots always moved randomly, the two others could fulfill the role of being either the chasing (wolf) or chased (sheep) dot. In the chase-present (but not the ...