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An improved harvest and in vitro expansion protocol for murine bone marrow-derived mesenchymal stem cells Vrije Universiteit Brussel

Song Xu, Ann De Becker, Benjamin Van Camp, Karin Vanderkerken, Ivan Van Riet
Compared to bone marrow (BM) derived mesenchymal stem cells (MSCs) from human origin or from other species, the in vitro expansion and purification of murine MSCs (mMSCs) is much more difficult because of the low MSC yield and the unwanted growth of non-MSCs in the in vitro expansion cultures. We describe a modified protocol to isolate and expand murine BM derived MSCs based on the combination of mechanical crushing and collagenase digestion at ...

In-feed resin acids reduce matrix metalloproteinase activity in the ileal mucosa of healthy broilers without inducing major effects on the gut microbiota Ghent University

Marisol Aguirre Morales, Juhani Vuorenmaa, Eija Valkonen, Hannele Kettunen, Chana Callens, Freddy Haesebrouck, Richard Ducatelle, Filip Van Immerseel, Evy Goossens
The chicken gut is constantly exposed to harmful molecules and microorganisms which endanger the integrity of the intestinal wall. Strengthening intestinal mucosal integrity is a key target for feed additives that aim to promote intestinal health in broilers. Recently, dietary inclusion of resin-based products has been shown to increase broiler performance. However, the mode of action is still largely unexplored. Coniferous resin acids are known ...

A simple procedure for the enzymic digestion of edible tissues prior to detection of drug residues Research Institute for Agriculture, Fisheries and Food

Els Daeseleire, A De Guesquière, C. Van Peteghem
A simple procedure for the enzymic digestion of edible tissues is described and compared with other procedures. The samples are digested overnight in an enzyme suspension containing subtilisin A at 60 degrees C and pH 9. The resulting digest contains only a few small tissue fragments. This method is suitable for routine analysis, since the manipulation of the samples is very limited.

Relationship between vaginal microbial dysbiosis, inflammation, and pregnancy outcomes in cervical cerclage KU Leuven

Christoph Lees
Preterm birth, the leading cause of death in children under 5 years, may be caused by inflammation triggered by ascending vaginal infection. About 2 million cervical cerclages are performed annually to prevent preterm birth. The procedure is thought to provide structural support and maintain the endocervical mucus plug as a barrier to ascending infection. Two types of suture material are used for cerclage: monofilament or multifilament braided. ...

Safety evaluation of the food enzyme microbial collagenase from the genetically modified Streptomyces violaceoruber strain pCol. Research Institute for Agriculture, Fisheries and Food

Lieve Herman
The food enzyme microbial collagenase (EC 3.4.24.3) is produced with the genetically modified Streptomyces violaceoruber strain pCol by Nagase (Europa) GmbH. The genetic modifications do not give rise to safety concerns. The food enzyme is free from viable cells of the production organism and its DNA. It is intended to be used in two food manufacturing processes: the production of modified meat and fish products and the production of protein ...