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Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

Tijdschriftbijdrage - Tijdschriftartikel

We expand photochromic super-resolution optical fluctuation imaging (pcSOFI) to monochromatic dual-channel sub-diffraction microscopy. Multi-tau (mt-)pcSOFI unmixes spectrally identical reversibly switchable fluorescent proteins (RSFPs) based on their blinking kinetics. We show that mt-pcSOFI can be used to simultaneously image two structures in living cells with existing RSFPs and the newly developed ffDronpa-F.
Tijdschrift: Chemical Communications
ISSN: 1359-7345
Issue: 53
Volume: 53
Pagina's: 7242 - 7245
Jaar van publicatie:2017
BOF-keylabel:ja
IOF-keylabel:ja
BOF-publication weight:2
CSS-citation score:1
Authors from:Higher Education
Toegankelijkheid:Open