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Split-BioID: a proximity-biotinylation assay for dimerization-dependent protein interactions

Tijdschriftbijdrage - Tijdschriftartikel

The biotin-identification (BioID) protocol uses a mutant of the biotin ligase BirA (BirA*) fused to a protein-of-interest to biotinylate proximate proteins in intact cells. Here, we show that two inactive halves of BirA* separately fused to a catalytic and regulatory subunit of protein phosphatase PP1 reconstitute a functional BirA* enzyme upon heterodimerization of the phosphatase subunits. We also demonstrate that this BirA*-fragment complementation approach, termed split-BioID, can be used to screen for substrates and other protein interactors of PP1 holoenzymes. Split-BioID is a novel and versatile tool for the identification of (transient) interactors of protein dimers. This article is protected by copyright. All rights reserved.
Tijdschrift: FEBS Letters
ISSN: 0014-5793
Issue: 2
Volume: 591
Pagina's: 415 - 424
Jaar van publicatie:2017
BOF-keylabel:ja
IOF-keylabel:ja
BOF-publication weight:1
CSS-citation score:2
Authors from:Higher Education
Toegankelijkheid:Open