Bone tissue response to BMP-2 adsorbed on amorphous microporous silica implants

Aim To evaluate bone regeneration potential of bone morphogenetic protein-2 (BMP-2) adsorbed on amorphous microporous silica (AMS). Materials & Methods Four implants [titanium as control (CTR); AMS-coated titanium (AMS), BMP-2 adsorbed on titanium (CTR+BMP) and AMS (AMS+BMP)] were implanted randomly in the tibiae of 20 New Zealand white rabbits. Bone specimens with implants were retrieved 2/4 weeks post implantation and analysed histologically and histomorphometrically. Bone fraction was measured in initial bone-free area (bone regeneration area, BRA) and in the area with initial bone–implant contact [bone adaptation area (BAA)] (BFBRA & BFBAA). Bone–implant contact was measured in BRA (BICBRA). In vitro BMP-2 release profiles were determined to evaluate the impact of the carrier surface. Mixed models were used for statistical analysis. Results BMP-2 release profiles were different for CTR+BMP and AMS+BMP. BICBRA and BFBRA were significantly increased after 4 weeks compared to 2 weeks for AMS, CTR+BMP and AMS+BMP. However, no differences between the implant types were observed within both healing periods. BFBAA for CTR+BMP was smaller than that for CTR and AMS+BMP after 4 weeks. Despite slower BMP-2 release, AMS+BMP did not stimulate bone regeneration. CTR+BMP caused bone resorption at the bone–implant interface. Conclusions BMP-2 functionalized implant surfaces failed to stimulate bone regeneration and osseointegration.

Jaar van publicatie:2012

BOF-keylabel:ja

IOF-keylabel:ja

BOF-publication weight:6

CSS-citation score:1

Authors from:Higher Education

Toegankelijkheid:Closed