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Comparison of fertilization rate and embryo quality after culture in embryo GPS™ or Primarie™ easy grip™ dishes.

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Introduction: The implementation of new tracking systems for embryo identification
in the IVF laboratories requires attachment of an ID tracking device onto
the culture dish. In order to introduce the ID system in our laboratory, the embryo
GPS(TM) dish (SunIVF®) was considered for oocyte and embryo culture. While
offering enough space for an ID tracking device, their design prevents media
droplets from collapsing, flattening, and mixing with each other. In addition,
the thinner plastic bottom provides improved heat exchange and visualisation.
A prospective sibling study was carried out to evaluate fertilization rate
and embryo quality during culture in the embryo GPS(TM) as compared to the
currently-used Primaria(TM) easy grip(TM) (Falcon®) dishes.
Material and Methods: In total, 47 cycles were included for the study. Inclusion
criteria were: ICSI cycles, fresh or frozen ejaculated sperm, ? 8 mature
oocytes. Embryo transfer was performed on day 3 or on day 5.
After injection, the sibling oocytes were randomly assigned to GPS(TM) or
Primaria(TM) dishes and cultured at 37°C, 6.0% CO2 and 5.0% O2 in sequential
Sage culture medium (CooperSurgical®). Fertilization was assessed 16-18h
after insemination by the presence of 2 pronuclei (PN) in the oocyte. Classification
of day 3 embryos was based on the number and symmetry of blastomeres,
percentage of fragmentation, vacuoles, granulation and multinucleation. Classification
of day 5 embryos was based on the criteria of Gardner and Schoolcraft
(1999). The overall embryo quality at the moment of transfer was classified into
4 categories: excellent (Q1), good (Q2), fair (Q3) and poor (Q4).
The Wilcoxon signed-rank test was used to compare the parameters studied
within the cycles.
Results: Out of 47 ICSI cycles included in the study, 260 injected oocytes were
cultured in GPS(TM) and 261 in Primaria(TM) dishes. No statistically significant difference
in fertilization rate was observed between GPS(TM) (85.9%) and Primaria(TM)
dishes (81.5%) (P = 0.12). The proportion of 1PN (2.41 % vs 3.13 %, P = 0.43)
or ? 3 PN (1.56 % vs 1.84%, P = 0.45) was also similar between the two groups.
No significant differences were observed between GPS(TM) vs Primaria(TM) for
the different embryo-quality parameters, neither on day 3 (70.28% vs 65.8%
Q1 + Q2, P = 0.25 and 27.9% vs 32.7% Q3 + Q4, P = 0.21, respectively) nor
on day 5 ET (28.5% vs 28% Q1 + Q2, P = 0.96 and 35.6% vs 35.6% Q3 + Q4,
P = 0.94, respectively).
Embryo transfer was performed on day 3 in 13 cycles, and on day 5 in
34 cycles. Always the best embryos were selected for transfers: only from the
GPS(TM) arm in 11 cycles, only from Primaria(TM) in 18 cycles, and mixed in 18
cycles. In both arms, a comparable number of supernumerary embryos were
cryopreserved.
Conclusions: The present preliminary validation indicates that embryo GPS(TM)
can replace Primaria(TM) easy grip dishes for fertilization and embryo culture.
However, a patient-to-patient randomisation trial is needed in order to assess
the pregnancy rate.
Tijdschrift: Hum Reprod
ISSN: 0268-1161
Volume: 26
Pagina's: 258, 355
Jaar van publicatie:2011
Trefwoorden:Embryo quality