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Untargeted metabolomics reveals elevated L‐carnitine metabolism in pig and rat colon tissue following red versus white meat intake

Tijdschriftbijdrage - e-publicatie

Scope The consumption of red and processed meat, and not white meat, has been associated with the development of various Western diseases such as colorectal cancer and type 2 diabetes. This work aimed at unravelling novel meat‐associated mechanisms that are involved in disease development. Methods and results A non‐hypothesis driven strategy of untargeted metabolomics was applied to assess colon tissue from rats (fed a high dose of beef versus white meat) and from pigs (fed red/processed meat versus white meat), receiving a realistic human background diet. An increased carnitine metabolism was observed, which was reflected by higher levels of acylcarnitines and 3‐dehydroxycarnitine (rats and pigs) and trimethylamine‐N‐oxide (rats). While 3‐dehydroxycarnitine was higher in HT29 cells, incubated with colonic beef digests, acylcarnitine levels were reduced. This suggested an altered response from colon cancer cell line towards meat‐induced oxidative stress. Moreover, metabolic differences between rat and pigs were observed in N‐glycolylneuraminic acid incorporation, prostaglandin and fatty acid synthesis. Conclusion This study demonstrated elevated (acyl)carnitine metabolism in colon tissue of animals that followed a red meat‐based diet, providing mechanistic insights that may aid in explaining the nutritional‐physiological correlation between red/processed meat and Western diseases.
Tijdschrift: Molecular nutrition and food research
ISSN: 1613-4125
Volume: 65
Jaar van publicatie:2021
Trefwoorden:A1 Journal article
Toegankelijkheid:Open