Fusion protein with a toxin and scaffold protein

The present invention relates to the field of structural biology and drug discovery. More specifically, the present invention relates to novel fusion proteins, their uses and methods in three-dimensional structural analysis of macromolecules, such as X-ray crystallography and high-resolution Cryo-EM, and their use in structure-based drug design and screening, and as pharmacological tools. Even more specifically, the invention relates to a functional fusion of a toxin and a scaffold protein wherein the folded scaffold protein interrupts the topology of the toxin by insertion in an exposed β-turn of a β-strand-containing domain of said toxin to form a rigid fusion protein that retains its high affinity target binding capacity.

Octrooi-publicatienummer: WO2020127993

Jaar aanvraag: 2020

Jaar toekenning: 2021

Gevalideerd voor IOF-sleutel: Ja

Toegewezen aan: Universitaire Associatie Brussel