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Octrooi

High-throughput genotyping by sequencing low amounts of genetic material

The present invention provides a method for analysis of target nucleic acids which are present in low amounts. In particular, the method comprises the following steps: i. providing a sample wherein target nucleic acids are present in a low amount, ii. generating a reduced representation library of said target nucleic acids by a method comprising: ⋅ fragmenting said target nucleic acids; ligating adaptors to said fragments; and selecting a subset of said adaptor-ligated fragments, iii. massively parallel sequencing said reduced representation library, and iv. identifying variants in said target nucleic acids by analyzing results obtained by said sequencing.
Octrooi-publicatienummer: EP2904113
Bron: EPO
Jaar aanvraag: 2013
Jaar aanvraag: 2016
Jaar van publicatie: 2020
Status: Assigned
Technologiedomeinen: Biotechnology
Gevalideerd voor IOF-sleutel: Ja
Toegewezen aan: Associatie KULeuven