Polynucleotide shuffling method

It is a first object of the present invention to provide a method for preparing a Tile vector, being a vector, which comprises a selectable marker and a coding polynucleotide, wherein said coding polynucleotide is immediately preceded and followed by a type Ms recognition sequence, wherein said preceding and following recognition sequences are recognized by a same type lis restriction enzyme, but have an opposite orientation. More particularly, the position and orientation of said preceding and following type lis recognition sequences provides for the cleavage of said Tile vector by a corresponding type lis restriction enzyme resulting in the release of said coding polynucleotide sequence having at its respective ends overhang sequences with a known orientation and length, while lacking said preceding and following type Ms recognition sequences. In a second object the present invention provides a method for using such Tile vectors obtained as previously described for joining two or more coding polynucleotides to form a product polynucleotide. Typically, said product polynucleotide is integrated in a vector.

Octrooi-publicatienummer: EP3555298

Jaar aanvraag: 2017

Jaar toekenning: 2020

Jaar van publicatie: 2019

Status: Aangevraagd

Technologiedomeinen: Biotechnologie

Gevalideerd voor IOF-sleutel: Ja

Toegewezen aan: Associatie KULeuven, Associatie Universiteit Gent