3D geprinte point-of-care diagnostiek gedreven door capillaire stroming
Point-of-care testing (POCT) is crucial for healthcare in developing countries, but remains
problematic. Among many, lateral flow devices (LFD) persist as the most promising, although theyoffer solutions only partially. This is mainly because they fail to accommodate complex bioassays
that are often needed to reach desired sensitivity. In this project, we aim to bridge this gap by
working on (i) the innovative 3D printing (3DP) of porous material for creating capillary flow-driven
microfluidics platform, (ii) understanding the bioassay kinetics in the porous matrix, (iii) inkjet
printing of biomolecules for their incorporation into the 3DPed platform and (iv) implementation
of a complex ELISA on the 3DPed platform. The proposed concept offers several key advantages.
First, 3DPed platform does not require any external devices for actuation of the fluids within the
channels, similar to LFD, but offers greater freedom in the assay design, due to the third
dimension. Second, inkjet printing of biomolecules directly into the device during fabrication
eliminates assembly steps from the traditional manufacturing. Third, the platform concept allows
on-chip pre-treatment of sample and the incorporation of semi-quantitative readout. Lastly, the
3DP is easily scalable and cost-effective approach, which is vital for POCT applications. Therefore,
the results obtained in this project will be extremely valuable both for scientists and clinicians
interested in the POCTs.