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Preliminary Characterization of the Transcriptional Response of the Porcine Intestinal Cell Line IPEC-J2 to Enterotoxigenic Escherichia coli, Escherichia coli, and E. coli Lipopolysaccharide KU Leuven

Marisa Geens, Theo Niewold
IPEC-J2, a promising in vitro model system, is not well characterized especially on the transcriptional level, in contrast to human counterparts. The aim of this study was to characterize the gene expression in IPEC-J2 cells when coincubated with enterotoxigenic Escherichia coli (ETEC), nonpathogenic E. coli, and E. coli endotoxin. Apical infection of polarized IPEC-J2 monolayers caused a time-dependent decrease in transepithelial electrical ...

Intra-ovarian growth factors regulating ovarian function in avian species: A review KU Leuven

Okanlawon Onagbesan, Veerle Bruggeman, Eddy Decuypere
There is now overwhelming evidence that the avian ovary is a site of production and action of several growth factors that have also been implicated in the functioning of the mammalian ovary. Several members of the Insulin-like growth factor family (IGF), the Epidermal growth factor family (EGF), the Transforming growth factor-beta family (TGF-beta), Fibroblast growth factors (FGF), the Tumour necrosis factor-alpha (TNF-alpha), and others, have ...

Direct comparison of hepatocyte-specific expression cassettes following adenoviral and nonviral hydrodynamic gene transfer KU Leuven

Frank Jacobs, Jan Snoeys, Yingmei Feng, Eline Van Craeyveld, J Lievens, D Armentano, SH Cheng, Bart De Geest
Hepatocytes are a key target for treatment of inborn errors of metabolism, dyslipidemia and coagulation disorders. The development of potent expression cassettes is a critical target to improve the therapeutic index of gene transfer vectors. Here we evaluated 22 hepatocyte-specific expression cassettes containing a human apo A-I transgene following hydrodynamic transfer of plasmids or adenoviral transfer with E1E3E4-deleted vectors in C57BL/6 ...