Publicaties
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Evidence of Cytolysin A nanopore incorporation in mammalian cells assessed by a graphical user interface KU Leuven
Technologies capable of assessing cellular metabolites with high precision and temporal resolution are currently limited. Recent developments in the field of nanopore sensors allow the non-stochastic quantification of metabolites, where a nanopore is acting as an electrical transducer for selective substrate binding proteins (SBPs). Here we show that incorporation of the pore-forming toxin Cytolysin A (ClyA) into the plasma membrane of Chinese ...
Quantitative determination of the full switching cycle of photochromic fluorescent proteins KU Leuven
In this study, we develop a general analytical model of the photochromism of fluorescent proteins and apply it to spectroscopic measurements performed on six different labels. Our approach provides quantitative explanations for phenomena such as the existence of positive and negative switching, limitations in the photochromism contrast, and the fact that initial switching cycles may differ from subsequent ones. It also allows us to perform the ...
Live-Cell SOFI Correlation with SMLM and AFM Imaging KU Leuven Universiteit Hasselt
Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry KU Leuven
Reversibly switchable monomeric Cherry (rsCherry) is a photoswitchable variant of the red fluorescent protein mCherry. We report that this protein gradually and irreversibly loses its red fluorescence in the dark over a period of months at 4 °C and a few days at 37 °C. We also find that its ancestor, mCherry, undergoes a similar fluorescence loss but at a slower rate. X-ray crystallography and mass spectrometry reveal that this is caused by the ...
An unusual disulfide-linked dimerization in the fluorescent protein rsCherryRev1.4 KU Leuven
rsCherryRev1.4 has been reported as one of the reversibly photoswitchable variants of mCherry, and is an improved version with a faster off-switching speed and lower switching fatigue at high light intensities than its precursor rsCherryRev. However, rsCherryRev1.4 still has some limitations such as a tendency to dimerize as well as complex photophysical properties. Here, the crystal structure of rsCherryRev1.4 was determined at a resolution of ...
Smart genetically-encoded biosensors for the chemical monitoring of living systems KU Leuven
Genetically-encoded biosensors provide the all-optical and non-invasive visualization of dynamic biochemical events within living systems, which has allowed the discovery of profound new insights. Twenty-five years of biosensor development has steadily improved their performance and has provided us with an ever increasing biosensor repertoire. In this feature article, we present recent advances made in biosensor development and provide a ...
Fluo: A Domain-Specific Language for Experiments in Fluorescence Microscopy (Application Paper) KU Leuven
Multilinear Slicing for curve resolution of fluorescence imaging with sequential illumination KU Leuven
Fluorescence microscopy is an extremely powerful technique that allows to distinguish multiple labels based on their emission color or other properties, such as their photobleaching and fluorescence recovery kinetics. These kinetics are ideally assumed to be mono-exponential in nature, where the time constants intrinsic to each fluorophore can be used to quantify their presence in the sample. However, these time constants also depend on the ...