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Opinion of organic and free-range pig farmers on animal welfare and the PIGLOW app for animal welfare self-assessments Universiteit Gent

Evelien Graat, Charlotte Vanden Hole, Mirjan Thys, Laura Warin, Anne Collin, Mona Giersberg, T Bas Rodenburg, Stella Nauta, Mathilde Stomp, Valérie Courboulay, et al.
The PIGLOW app was designed for the PPILOW project, enabling organic and free-range pig farmers to monitor the welfare of their pigs. The app is based on the 4 principles of the Welfare Quality protocol: good housing, good feeding, good health and appropriate behaviour. The tool includes automated feedback and anonymous benchmarking. A longitudinal study on 20-30 pig farms has started in order to determine the long-term effect of the use of the ...

Expression and putative function of fibronectin and its receptor (integrin alpha-5 beta-1) in male and female gametes during bovine fertilization in vitro Universiteit Gent

Mirjan Thys, Hans Nauwynck, Dominiek Maes, Maarten Hoogewijs, Dries Vercauteren, Tom Rijsselaere, Herman Favoreel, Ann Van Soom
Fibronectin (Fn) is a 440 kDa glycoprotein assumed to participate in sperm-egg interaction in human. Recently, it has been demonstrated that Fn - when present during bovine IVF - strongly inhibits sperm penetration. The present study was conducted firstly to evaluate the expression of Fn and its integrin receptor (alpha(5)beta(1)) on male and female bovine gametes using indirect immunofluorescence and secondly, to determine the function of Fn ...

In vitro fertilizing capacity of frozen-thawed bull spermatozoa selected by single-layer (glycidoxypropyltrimethoxysilane) silane-coated silica colloidal centrifugation Universiteit Gent

Mirjan Thys, Leen Vandaele, Jane M Morrell, Johanna Mestach, Ann Van Soom, Maarten Hoogewijs, Heriberto Rodriguez-Martinez
Contents Barriers to the use of density gradient centrifugation for preparing animal spermatozoa for artificial insemination (AI) include the scarcity of animal-specific formulations and the daunting prospect of processing large volumes of ejaculate in small aliquots (1.5 ml extended semen). Recently, new colloid formulations have been tested in vitro in a modified procedure, centrifugation on a single layer of colloid. The present study ...