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Tweede lichenologisch bezoek van de WBL aan het Kollintenbos en het Bos van Aa op 17/11/2018 Plantentuin Meise / Agentschap Plantentuin Meise
It’s the second time that the WBL has visited the protected nature reserves ‘Forest of Aa’ and ‘Kollinten Forest’. The first time was on 2 April 2011 but the results of this excursion were not yet published. In this article together the results of both excursions are presented. The following noteworthy species are discussed in detail: Alyxoria viridipruinosa, Athallia pyracea, Chaenotheca brachypoda, Cladonia cariosa, Macentina stigonemoides, ...
Een derde lichenologisch bezoek aan het Bos van Aa te Zemst-Laar door de WBL Plantentuin Meise / Agentschap Plantentuin Meise
After previous inventories in 2011 and 2018, the Forest of Aa was re-investigated on the 14th of July 2019 for lichens and lichenicolous fungi and this for the third time. Eleven participants attended this excursion. We focused on the western grasslands, the central swamp and the southern part of the swamp forest. During the previous two excursions we were able to record a total of 79 taxa (71 lichens and 8 lichenicolous fungi). Now 21 species ...
Muscling through AAV immunity. Vrije Universiteit Brussel
AAV-1 is one of the most promising vectors for gene delivery to skeletal muscle. In this issue of Blood, Mingozzi and colleagues now demonstrate that AAV-1-mediated gene transfer into human skeletal muscle results in activation of capsid-specific T cells.
Intraparenchymal convection enhanced delivery of AAV in sheep to treat Mucopolysaccharidosis IIIC KU Leuven
BACKGROUND: Mucopolysaccharidosis IIIC (MPSIIIC) is one of four Sanfilippo diseases sharing clinical symptoms of severe cognitive decline and shortened lifespan. The missing enzyme, heparan sulfate acetyl-CoA: α-glucosaminide-N-acetyltransferase (HGSNAT), is bound to the lysosomal membrane, therefore cannot cross the blood-brain barrier or diffuse between cells. We previously demonstrated disease correction in MPSIIIC mice using an ...
Three-dimensional multipotent progenitor cell aggregates for expansion, osteogenic differentiation and 'in vivo' tracing with AAV vector serotype 6 KU Leuven
Multipotent adult progenitor cells (MAPCs) are bone marrow-derived stem cells with a high growth rate suitable for therapeutical applications as three-dimensional (3D) aggregates. Combined applications of osteogenically differentiated MAPC (OD-MAPC) aggregates and adeno-associated viral vectors (AAV) in bone bioengineering are still deferred until information with regard to expansion technologies, osteogenic potential, and AAV cytotoxicity and ...
Identification of a myotropic AAV by massively parallel in vivo evaluation of barcoded capsid variants KU Leuven Vrije Universiteit Brussel
Adeno-associated virus (AAV) forms the basis for several commercial gene therapy products and for countless gene transfer vectors derived from natural or synthetic viral isolates that are under intense preclinical evaluation. Here, we report a versatile pipeline that enables the direct side-by-side comparison of pre-selected AAV capsids in high-throughput and in the same animal, by combining DNA/RNA barcoding with multiplexed next-generation ...
Semirational bioengineering of AAV vectors with increased potency and specificity for systemic gene therapy of muscle disorders KU Leuven Vrije Universiteit Brussel
Bioengineering of viral vectors for therapeutic gene delivery is a pivotal strategy to reduce doses, facilitate manufacturing, and improve efficacy and patient safety. Here, we engineered myotropic adeno-associated viral (AAV) vectors via a semirational, combinatorial approach that merges AAV capsid and peptide library screens. We first identified shuffled AAVs with increased specificity in the murine skeletal muscle, diaphragm, and heart, ...