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Assessment of IgA anti-PT and IgG anti-ACT reflex testing to improve Bordetella pertussis serodiagnosis in recently vaccinated subjects Vrije Universiteit Brussel

Lorenzo Subissi, C Rodeghiero, Helena Martini, A Litzroth, Kimberley Huygen, Denis Pierard, Isabelle Desombere

OBJECTIVES: Quantifying IgG antibodies to pertussis toxin (PT) is the most specific and sensitive method for the serodiagnosis of a Bordetella pertussis infection. Since PT is a component of acellular pertussis vaccines, anti-PT IgG is also induced by vaccination, precluding pertussis serodiagnosis based exclusively on anti-PT IgG in recently vaccinated subjects. Here, we aim to identify additional B. pertussis-specific serological markers ...

Improving specificity of Bordetella pertussis detection using a four target real-time PCR Vrije Universiteit Brussel Universiteit Antwerpen

Helena Martini, Liselot Detemmerman, Oriane Soetens, Erlangga Yusuf, Denis Pierard

The incidence of whooping cough, a contagious respiratory disease caused by Bordetella pertussis, is on the rise despite existing vaccination programmes. Similar, though usually milder, respiratory symptoms may be caused by other members of the Bordetella genus: B. parapertussis, B. holmesii, and B. bronchiseptica. Pertussis diagnosis is mostly done using PCR, but the use of multiple targets is necessary in order to differentiate the ...

Surveillance of Circulating Bordetella pertussis Strains in Europe during 1998 to 2015 Vrije Universiteit Brussel

Jussi Mertsola, Denis Pierard, Silje Vermedal Hoegh, Sophie Guillot, Paola Stefanelli, Marjolein Van Gent, Didrik F Vestrheim

One reason for increased pertussis incidence is the adaptation of Bordetella pertussis to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 (n=265) and compares the results to previous EUpert I-III studies (1998-2009). The analyses included genotyping, serotyping and pulsed-field gel electrophoresis (PFGE) and multi-locus ...

Both CD4⁺ and CD8⁺ lymphocytes participate in the IFN-γ response to filamentous hemagglutinin from Bordetella pertussis in infants, children, and adults. Vrije Universiteit Brussel

V. Dirix, Virginie Verscheure, F. Vermeulen, Iris De Schutter, T. Goetghebuer, C. Locht, F. Mascart
Infant CD4(+) T-cell responses to bacterial infections or vaccines have been extensively studied, whereas studies on CD8(+) T-cell responses focused mainly on viral and intracellular parasite infections. Here we investigated CD8+ T-cell responses upon Bordetella pertussis infection in infants, children, and adults and pertussis vaccination in infants. Filamentous hemagglutinin-specific IFN-gamma secretion by circulating lymphocytes was blocked ...

Bordetella pertussis seroprevalence in Belgian adults aged 20-39 years, 2012. Vrije Universiteit Brussel

K. Huygen, C Rodeghiero, D Govaerts, I Leroux-Roels, P. Melin, M. Reynders, Sam Vander Meeren, S. Vandenwijngaert, Denis Pierard
SUMMARY The last report on pertussis seroprevalence in Belgium concerned samples collected during 1993-1994. In the context of the Eupert-Labnet WP6 seroprevalence study (comparing sera from 16 European member states), 1500 anonymized leftover diagnostic samples were collected randomly during the second semester of 2012 by the clinical chemistry laboratories of six participating Belgian centres, distributed equally between Flanders, Wallonia and ...

Bordetella pertussis seroprevalence in Belgian adults aged 20-39 years, 2012 Universiteit Gent

K Huygen, C Rodeghiero, D Govaerts, Isabel Leroux-Roels, P Melin, M Reynders, S Van der Meeren, S Van den Wijngaert, D Pierard
The last report on pertussis seroprevalence in Belgium concerned samples collected during 1993-1994. In the context of the Eupert-Labnet WP6 seroprevalence study (comparing sera from 16 European member states), 1500 anonymized leftover diagnostic samples were collected randomly during the second semester of 2012 by the clinical chemistry laboratories of six participating Belgian centres, distributed equally between Flanders, Wallonia and ...

Assessing the reactogenicity of Tdap vaccine administered during pregnancy and antibodies to Bordetella pertussis antigens in maternal and cord sera of Thai women Universiteit Hasselt Universiteit Antwerpen

Nasamon Wanlapakorn, Kirsten Maertens, Surasith Chaithongwongwatthana, Donchida Srimuan, Narissara Suratannon, Sompong Vongpunsawad, Mai Phuong Thao TRAN, Niel HENS, Pierre Van Damme, Camille Locht, et al.
Introduction: Pregnant Thai women have low antibody titers against B. pertussis antigens, which coincide with an increasing incidence of pertussis among Thai infants. Thus, there exists a potential benefit of a booster dose of tetanus- diphtheria-acellular pertussis (Tdap) vaccine administered during pregnancy. Here, we report the vaccine reactogenicity profile and birth outcomes in Tdap-vaccinated pregnant women who have or have not had prior ...

Antibodies to Bordetella pertussis antigens in maternal and cord blood pairs: a Thai cohort study Universiteit Antwerpen

Nasamon Wanlapakorn, Thanunrat Thongmee, Preeyaporn Vichaiwattana, Elke Leuridan, Sompong Vongpunsawad, Yong Poovorawan
Background. Pertussis is a vaccine-preventable disease, yet an increasing incidence of pertussis occurs in many countries. Thailand has a long-standing pertussis vaccination policy, therefore most expectant mothers today had received vaccines as children. The resurgence of pertussis among Thai infants in recent years led us to examine the preexisting antibodies to Bordetella pertussis antigens in a cohort of 90 pregnant women. Methods. We ...