Publicaties
Cleavable Linkers in Chemical Proteomics Applications KU Leuven
Development of a new microwave-assisted cleavable backbone amide linker (BAL): a comparative study KU Leuven
Development of a new microwave-assisted cleavage backbone amide linker (BAL): a comparative study. Vrije Universiteit Brussel
Reducing the renal retention of low- to moderate-molecular-weight radiopharmaceuticals Vrije Universiteit Brussel
Labeling a TCO-functionalized single domain antibody fragment with F-18 via inverse electron demand Diels Alder cycloaddition using a fluoronicotinyl moiety-bearing tetrazine derivative Vrije Universiteit Brussel
Single domain antibody fragments (sdAbs) exhibit a rapid tumor uptake and fast blood clearance amenable for labeling with 18F (t½ = 110 min) but suffer from high kidney accumulation. Previously, we developed a method for 18F-labeling of sdAbs via trans-cyclooctene (TCO)-tetrazine (Tz) inverse electron demand Diel's Alder cycloaddition reaction (IEDDAR) that incorporated a renal brush border enzyme (RBBE)-cleavable linker. Although >15 fold ...
An Efficient Method for Labeling Single Domain Antibody Fragments with 18F Using Tetrazine- Trans-Cyclooctene Ligation and a Renal Brush Border Enzyme-Cleavable Linker Vrije Universiteit Brussel
Single domain antibody fragments (sdAbs) labeled with 18F have shown promise for assessing the status of oncological targets such as the human epidermal growth factor receptor 2 (HER2) by positron emission tomography (PET). Earlier, we evaluated two residualizing prosthetic agents for 18F-labeling of anti-HER2 sdAbs; however, these methods resulted in poor labeling yields and high uptake of 18F activity in the kidneys. To potentially mitigate ...