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Taxonomic challenges in freshwater fishes: a mismatch between morphology and DNA barcoding in fish of the north-eastern part of the Congo basin KU Leuven Universiteit Antwerpen
This study evaluates the utility of DNA barcoding to traditional morphology-based species identifications for the fish fauna of the north-eastern Congo basin. We compared DNA sequences (COI) of 821 samples from 206 morphologically identified species. Best match, best close match and all species barcoding analyses resulted in a rather low identification success of 87.5%, 84.5% and 64.1%, respectively. The ratio 'nearest-neighbour distance/maximum ...
DNA barcoding the Lake Edward basin: high taxonomic coverage of a tropical freshwater ichthyofauna KU Leuven
DNA barcoding to improve the taxonomy of the Afrotropical hoverflies (Insecta: Diptera: Syrphidae) Universiteit Antwerpen
The identification of Afrotropical hoverflies is very difficult because of limited recent taxonomic revisions and the lack of comprehensive identification keys. In order to assist in their identification, and to improve the taxonomy of this group, we constructed a reference dataset of 513 COI barcodes of 90 of the more common nominal species from Ghana, Togo, Benin and Nigeria (W Africa) and added ten publically available COI barcodes from nine ...
A time-calibrated multi-gene phylogeny provides insights into the evolution, taxonomy and DNA barcoding of the Pinnularia gibba group (Bacillariophyta ) Universiteit Gent Plantentuin Meise / Agentschap Plantentuin Meise
Many diatom groups are known for widespread (pseudo)cryptic species diversity and Pinnularia gibba group is one of them. Recently, KoLLAR et al. (2019) delimited species within the group by means of a polyphasic approach, providing an evidence for the existence of fifteen species. In order to further guide the systematic revision of the group, the present study focuses on the evolution and morphology of the P. gibba group. Using representatives ...
Identification of Belgian mosquito species (Diptera: Culicidae) by DNA barcoding Instituut voor Tropische Geneeskunde Universiteit Antwerpen
Since its introduction in 2003, DNA barcoding has proven to be a promising method for the identification of many taxa, including mosquitoes (Diptera: Culicidae). Many mosquito species are potential vectors of pathogens, and correct identification in all life stages is essential for effective mosquito monitoring and control. To use DNA barcoding for species identification, a reliable and comprehensive reference database of verified DNA ...
Morphometry and DNA barcoding reveal cryptic diversity in the genus Enteromius (Cypriniformes: Cyprinidae) from the Congo basin, Africa KU Leuven Universiteit Antwerpen
© 2017, Museum National d’Histoire Naturelle. All rights reserved. One of the main challenges to adequately conserve the African fish fauna is to improve our so far unsatisfactory taxonomic knowledge of important portions of the ichthyofauna. In the present study, we attempted to unravel the taxonomic diversity of some species of Enteromius Cope, 1867, a problematic African fish genus, recently collected in the north-eastern part of the Congo ...
DNA barcoding fishes from the Congo and the Lower Guinean provinces Universiteit Antwerpen KU Leuven
The Congolese and Lower Guinean ichthyological provinces are understudied hotspots of the global fish diversity. Here, we barcoded 741 specimens from the Lower and Middle Congo River and from three major drainage basins of the Lower Guinean ichthyological province, Kouilou‐Niari, Nyanga and Ogowe. We identified 194 morphospecies belonging to 82 genera and 25 families. Most morphospecies (92.8%) corresponded to distinct clusters of DNA barcodes. ...
Systematics and DNA barcoding of free-living marine nematodes with emphasis on tropical desmodorids using nuclear SSU rDNA and mitochondrial COI sequences Instituut voor Landbouw-, Visserij- en Voedingsonderzoek Universiteit Gent Universiteit Antwerpen
The diversity and phylogenetic relationships of the Desmodoridae, a widespread tropical family of free-living marine nematodes, is hitherto poorly known both from molecular and taxonomic points of view. We performed a molecular phylogenetic analysis of marine nematodes to: i) disentangle relationships among tropical desmodorid species; and ii) compare the performance of the nuclear SSU rDNA and mitochondrial COI nucleotide sequences in 42 and 45 ...
DNA barcoding of amphibians and reptiles Universiteit Antwerpen
Only a few major research programs are currently targeting COI barcoding of amphibians and reptiles (including chelonians and crocodiles), two major groups of tetrapods. Amphibian and reptile species are typically old, strongly divergent, and contain deep conspecific lineages which might lead to problems in species assignment with incomplete reference databases. As far as known, there is no single pair of COI primers that will guarantee a ...