Publicaties

Grapevine development and ripening are complex processes that involve several biochemical pathways, including fatty acid and lipid metabolism. Fatty acids are essential components of lipids, which play crucial roles in fruit maturation and flavor development. However, the dynamics of fatty acid metabolism in grape flowers and berries are poorly understood. In this study, we present those dynamics and investigate the mechanisms of fatty acid ...

In order to study the effect of grazing pastures with a different botanical composition on rumen and intramuscular fatty acid metabolism, 21 male lambs were assigned to three botanically different pastures: botanically diverse (BD) (consisting for 65% of a variety of grass species); Leguminosa rich (L) (consisting for 61% of Leguminosae) and intensive English ryegrass (IR) (with 69% Lolium perenne). Pastures were sampled weekly for 12 weeks for ...

In the first experiment of this research, four dietary lipid sources (palm fat, P; soybean oil, S; linseed oil, L; fish oil, F) were added at 3% to a basal diet containing 5% palm fat. Diets were fed to female and male birds from d 1 to either d 21 or d 42 of age. Birds were slaughtered at d 21 or 42 of the experiment, tissues were excised and fatty acid composition was determined in seven anatomical compartments. The birds fed the L diet showed ...

To study the effect of feeding silages with different botanical composition, on rumen and lamb fat, 30 male lambs were assigned to five different silage groups for 11 weeks: botanically diverse silage (BDS); white clover silage (WCS); red clover silage (RCS), intensive English ryegrass silage (IRS) and crushed linseed and maize silage (MSL). Besides the silages, animals received organic wheat and barley and the MSL group additionally received ...

Effects of different inclusion levels of oil palm fronds (OPF) on rumen fermentation, apparent biohydrogenation of linoleic (C18:2n-6) and linolenic (C18:3n-3) acid and their biohydrogenation intermediates are described after 24 h in vitro incubations with buffer (20 ml), rumen fluid (5 ml) and standard dairy concentrate (0.250, 0.225, 0.200 and 0.175 g). Four inclusion levels of OPF (0, 0.025, 0.050 and 0.075 g) were tested in two in vitro ...

In high-yielding dairy cows, some fertility traits can be influenced by the fatty acid (FA) composition of the follicular fluid during early lactation. The first objective of the current study was to evaluate the potential of dietary supplements enriched in specific FA to influence the FA composition of follicular fluid lipid classes in early lactation dairy cows. The second objective was to determine the influence of the resulting follicular ...

White and red clover silages have been shown to result in a higher transfer efficiency of polyunsaturated fatty acids (PUFA) to the milk or meat of ruminants compared to ryegrass silages. For red clover this has been related to protein bound phenols formed by polyphenol oxidase (PPO). PPO is typically active in red clover and the protein bound phenols, which are the result of PPO activity, could result in a decrease in PUFA biohydrogenation in ...

The fatty acid (FA) concentration of herbage and lipid metabolism in silage, mainly oxidation and lipolysis, of different species (perennial ryegrass, red clover and white clover) and three cultivars of white and red clover at three cutting dates in the growing season (April, July and October) were studied. FA concentration and composition was strongly affected by species and cutting date. Perennial ryegrass had lower concentrations of C16:1, ...

Acyl-CoA thioesterase (ACOT) activities are found in prokaryotes and in several compartments of eukaryotes where they hydrolyze a wide range of acyl-CoA substrates and thereby regulate intracellular acyl-CoA/CoA/fatty acid levels. ACOT9 is a mitochondrial ACOT with homologous genes found from bacteria to humans and in this study we have carried out an in-depth kinetic characterization of ACOT9 to determine its possible physiological function. ...