Method for the in vitro differentiation and maturation of dendritic cells for therapeutic use Universiteit Gent
High yields of fully-differentiated mature dendritic cells can be obtained by a shortened monocyte culture protocol involving maturation with a TLR4-ligand plus IFN-γ The feasibility of generating DCs by combining a greatly reduced monocyte culture duration, together with maturation using an established type-1 polarizing factor combination, was assessed using an extensive series of small-scale cultures starting from buffy coats. Cell culture media, cytokines and closed-system containers were selected for direct translation to our GMP production environment. To reduce the need for operator ...